Cryopreservation of enucleated human neutrophils (PMN cytoplasts)
نویسندگان
چکیده
منابع مشابه
Uptake of antibiotics by human polymorphonuclear leukocyte cytoplasts.
Enucleated human polymorphonuclear leukocytes (PMN cytoplasts), which have no nuclei and only a few granules, retain many of the functions of intact neutrophils. To better define the mechanisms and intracellular sites of antimicrobial agent accumulation in human neutrophils, we studied the antibiotic uptake process in PMN cytoplasts. Entry of eight radiolabeled antibiotics into PMN cytoplasts w...
متن کاملFunctional activity of enucleated human polymorphonuclear leukocytes
Enucleated human polymorphonuclear leukocytes (PMN) were prepared by centrifuging isolated, intact PMN over a discontinuous Ficoll gradient that contained 20 microM cytochalasin B. The enucleated cells (PMN cytoplasts) contained about one-third of the plasma membrane and about one-half of the cytoplasm present in intact PMN. The PMN cytoplasts contained no nucleus and hardly any granules. The v...
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The neutrophil respiratory burst, which is essential for the host's ability to kill microorganisms, can be stimulated by some natural agents. Olea europaea (Oleaceae) is used as anti hypertensive, hyperglycemia, and hypercholestremia in traditional medicine. Here in this study, we evaluate the stimulatory effect of olive oil extract on oxidative burst in human neutrophils in vitro. Buffy coat w...
متن کاملThe effect of olive extract on human neutrophils
The neutrophil respiratory burst, which is essential for the host's ability to kill microorganisms, can be stimulated by some natural agents. Olea europaea (Oleaceae) is used as anti hypertensive, hyperglycemia, and hypercholestremia in traditional medicine. Here in this study, we evaluate the stimulatory effect of olive oil extract on oxidative burst in human neutrophils in vitro. Buffy coat w...
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Actinobacillus actinomycetemcomitans leukotoxin lysed human neutrophil cytoplasts. The reaction was associated with a rapid influx of extracellular calcium, a collapse in membrane potential, release of lactate dehydrogenase, and overt disintegration of the plasma membrane. These functional and structural alterations in the plasmalemma of neutrophil cytoplasts reinforce the hypothesis that A. ac...
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ژورنال
عنوان ژورنال: Blood
سال: 1984
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v63.1.234.234